Jahagirdar A P, Downer R G, Viswanatha T
Biochim Biophys Acta. 1984 Sep 28;801(2):177-83. doi: 10.1016/0304-4165(84)90065-5.
Injection of adult male cockroaches (Periplaneta americana) with 10 microliter 1 microM octopamine causes elevated activity of trehalase (alpha, alpha-trehalose glucohydrolase; EC 3.2.1.28) in hemolymph and muscle but not in gut. Tyramine, dopamine and glutamate, at the same concentration, failed to elicit any effect on trehalase activity. Determination of some kinetic parameters for muscle and hemolymph trehalases reveal that octopamine causes an increase in Vmax without any significant alteration in the Km of the enzyme for trehalose. The results are discussed in terms of the physiological significance of octopamine-mediated activation of tissue trehalases.
给成年雄性蟑螂（美洲大蠊）注射10微升1微摩尔的章鱼胺，会使血淋巴和肌肉中的海藻糖酶（α,α-海藻糖葡糖苷水解酶；EC 3.2.1.28）活性升高，但肠道中的海藻糖酶活性不受影响。相同浓度的酪胺、多巴胺和谷氨酸对海藻糖酶活性没有任何影响。对肌肉和血淋巴中海藻糖酶的一些动力学参数进行测定后发现，章鱼胺会使Vmax增加，而酶对海藻糖的Km没有显著变化。本文根据章鱼胺介导的组织海藻糖酶激活的生理意义对结果进行了讨论。
