Murata K, Kudo M, Onuma F, Motoyama T
Hepatogastroenterology. 1984 Aug;31(4):158-61.
Macromolecular collagen components in normal liver and at the different stages of human liver cirrhosis were studied under various extraction conditions. The collagen content at the typical stage of liver cirrhosis was more than five-fold higher than that of the normal state. Pepsin-solubilized collagens extracted successively accounted for 90% of the total collagen and were subjected to determination of the collagen types by salt differentiated fractionation and SDS-polyacrylamide gel electrophoresis. Both type I and III collagens, especially the former, increased, reflecting enhanced total collagen with the progression of liver cirrhosis. The ratio of type I to type III was 1.02 - 1.22 in normal liver and at the early stage of liver cirrhosis, but increased to 1.58 and 1.60 at the typical and advanced stages of liver cirrhosis, respectively. At the early stage, the remarkable increase in type V collagen started much earlier than at the typical stage when the ratio of type I to type III changed. The enhancement of type V collagen may result from a cell proliferative phenomenon at the earlier stage of liver cirrhosis.
在不同提取条件下，对正常肝脏以及人类肝硬化不同阶段的大分子胶原成分进行了研究。肝硬化典型阶段的胶原含量比正常状态高出五倍多。依次提取的胃蛋白酶可溶解胶原占总胶原的90%，并通过盐分级分离和SDS-聚丙烯酰胺凝胶电泳对胶原类型进行测定。I型和III型胶原，尤其是前者，均增加，这反映出随着肝硬化的进展，总胶原含量增加。正常肝脏和肝硬化早期I型与III型胶原的比例为1.02 - 1.22，但在肝硬化典型期和晚期分别增至1.58和1.60。在早期，V型胶原的显著增加比I型与III型胶原比例变化的典型阶段要早得多。V型胶原的增加可能是肝硬化早期细胞增殖现象所致。
