Begue J M, Guguen-Guillouzo C, Pasdeloup N, Guillouzo A
Hepatology. 1984 Sep-Oct;4(5):839-42. doi: 10.1002/hep.1840040507.
The cytochrome P-450 content promptly fell in adult rat hepatocytes cultured in conventional conditions whereas no obvious change was detected over a 10-day period when these cells were co-cultured with another rat liver epithelial cell type. A concomitant maintenance of the amino-pyrine N-demethylase activity and a high aflatoxin B1-induced cytotoxicity was observed, and addition of phenobarbital to the culture medium produced about a 2-fold increase in cytochrome P-450 level. These results indicate that when placed in co-culture, adult hepatocytes remain differentiated for several days and suggest that such a co-culture system is an appropriate model for pharmacotoxicological studies.
在常规条件下培养的成年大鼠肝细胞中，细胞色素P - 450含量迅速下降，而当这些细胞与另一种大鼠肝上皮细胞类型共培养时，在10天的时间内未检测到明显变化。观察到氨基比林N - 脱甲基酶活性的同时维持以及黄曲霉毒素B1诱导的高细胞毒性，并且向培养基中添加苯巴比妥使细胞色素P - 450水平提高了约2倍。这些结果表明，成年肝细胞在共培养时可保持分化状态数天，提示这种共培养系统是药物毒理学研究的合适模型。
