Laszlo D J, Niwano M, Goral W W, Taylor B L
J Bacteriol. 1984 Sep;159(3):820-4. doi: 10.1128/jb.159.3.820-824.1984.
Aerotaxis (migration towards oxygen) of Bacillus cereus M63, a motile strain, was inhibited by potassium cyanide and 2-heptyl-4-hydroxyquinoline N-oxide, indicating a requirement for both the terminal oxidase (cytochrome aa3) and the cytochrome b segment of the electron transport system. The concentration of oxygen that gave a half-maximal aerotactic response (K0.5) was 0.31 microM, which was similar to the Km for respiration (0.80 microM). The proton motive force increased from -135 to -177 mV when anaerobic cells were aerated, and it is proposed that the signal for aerotaxis is the increase in proton motive force that results from increased respiration. A strain of B. cereus T initially used in this study was immotile, grew as long chains of cells, and was deficient in autolytic enzyme. B. cereus M63 is a spontaneous derivative of B. cereus T that has normal motility.
蜡样芽孢杆菌M63是一种运动性菌株，其趋氧性（向氧迁移）受到氰化钾和2-庚基-4-羟基喹啉N-氧化物的抑制，这表明电子传递系统的末端氧化酶（细胞色素aa3）和细胞色素b片段均不可或缺。产生半数最大趋氧反应（K0.5）的氧气浓度为0.31微摩尔，这与呼吸作用的米氏常数（0.80微摩尔）相似。当厌氧细胞通气时，质子动力势从-135毫伏增加到-177毫伏，并且有人提出趋氧性的信号是呼吸作用增强导致的质子动力势增加。本研究最初使用的蜡样芽孢杆菌T菌株不具有运动性，以长链细胞形式生长，并且缺乏自溶酶。蜡样芽孢杆菌M63是蜡样芽孢杆菌T的自发衍生物，具有正常的运动性。
