Biely P, Petráková E
J Bacteriol. 1984 Oct;160(1):408-12. doi: 10.1128/jb.160.1.408-412.1984.
A series of compounds structurally related to xylan and 1,4-beta-xylobiose were tested as inducers of the xylan-degrading enzyme system of Cryptococcus albidus. Washed, glucose-grown cells were incubated with alpha- and beta-linked xylobioses, 4-O-beta-D-xylopyranosyl-L-arabinopyranose, 3-O-beta-D-xylopyranosyl-xylobiose, 6-O-beta-D-xylopyranosyl-cellobiose, cellobiose, and methyl beta-D-xylopyranoside. All alpha-xylobioses and cellobiose were inactive as inducers of the xylan-degrading enzyme system. Other compounds served as inducers of varying efficiency, depending on their concentration in the induction medium and the time of incubation of cells. The most rapid response of the cells, i.e., the shortest induction period of beta-xyloside permease, beta-xylosidase (EC 3.2.1.37), and beta-xylanase (EC 3.2.1.8), was observed with 1,4-beta-xylobiose, which was the most efficient inducer at low concentrations (0.1 to 0.2 mM). At higher concentrations (2 to 10 mM) and after long incubations, the highest enzyme yields were obtained with 1,2-beta-xylobiose. The results represent a new example of efficient induction of polysaccharide-degrading enzyme systems by positional isomers of dimers derived from the polysaccharide.
测试了一系列与木聚糖和1,4-β-木二糖结构相关的化合物作为浅白隐球酵母木聚糖降解酶系统的诱导剂。将用葡萄糖培养的洗涤过的细胞与α-和β-连接的木二糖、4-O-β-D-吡喃木糖基-L-阿拉伯吡喃糖、3-O-β-D-吡喃木糖基-木二糖、6-O-β-D-吡喃木糖基-纤维二糖、纤维二糖和甲基β-D-吡喃木糖苷一起孵育。所有α-木二糖和纤维二糖作为木聚糖降解酶系统的诱导剂均无活性。其他化合物作为诱导剂的效率各不相同，这取决于它们在诱导培养基中的浓度以及细胞孵育的时间。用1,4-β-木二糖观察到细胞的最快反应，即β-木糖苷通透酶、β-木糖苷酶（EC 3.2.1.37）和β-木聚糖酶（EC 3.2.1.8）的最短诱导期，1,4-β-木二糖在低浓度（0.1至0.2 mM）下是最有效的诱导剂。在较高浓度（2至10 mM）和长时间孵育后，用1,2-β-木二糖获得最高的酶产量。这些结果代表了由多糖衍生的二聚体的位置异构体有效诱导多糖降解酶系统的一个新例子。
