Dlugi A M, Laufer N, Polan M L, DeCherney A H, Tarlatzis B C, MacLusky N J, Behrman H R
J Clin Endocrinol Metab. 1984 Nov;59(5):986-92. doi: 10.1210/jcem-59-5-986.
The production of 17 beta-estradiol and progesterone (Prog) by human granulosa-luteal cells obtained from 24 aspirated follicles of 11 women undergoing laparoscopy in an in vitro fertilization program was studied. Follicular growth was stimulated with an individualized human menopausal gonadotropin regimen begun on either day 1 (group I; n = 5) or day 3 (group II; n = 6) of the menstrual cycle, and laparoscopy was performed 36 h after hCG administration. Granulosa-luteal cells were cultured for 2 h in culture medium alone or in the presence of either pregnenolone (10(-7) M) or testosterone (10(-7) M). Aromatase activity was present in the granulosa-luteal cells, as evidenced by a significant (P less than 0.001) increase in E2 production in the presence of testosterone. The addition of pregnenolone did not augment Prog production. Granulosa-luteal cells derived from Group II patients produced significantly (P less than 0.001) more Prog than those derived from group I patients. In addition, group II granulosa-luteal cells associated with mature oocyte-coronacumulus complexes produced significantly (P less than 0.001) more Prog than those in group I. Fertilization and pregnancy correlated with Prog production, in that granulosa-luteal cells associated with oocytes that were fertilized produced significantly (P less than 0.001) less Prog than those associated with nonfertilized oocytes. Granulosa-luteal cells from the 2 patients in this series who conceived demonstrated a further significant (P less than 0.02) reduction in Prog production. It appears that administration of human menopausal gonadotropin early (day 1) in the follicular phase results in incomplete maturation of the granulosa cells. Furthermore, the optimal oocyte, in terms of successful fertilization, may be one derived from a follicle that has undergone appropriate stimulation resulting in adequate maturation, but has not surpassed that point.
对11名接受体外受精计划腹腔镜检查的女性的24个抽吸卵泡中获取的人颗粒黄体细胞产生17β-雌二醇和孕酮（Prog）的情况进行了研究。在月经周期的第1天（I组；n = 5）或第3天（II组；n = 6）开始采用个体化的人绝经期促性腺激素方案刺激卵泡生长，并在注射hCG后36小时进行腹腔镜检查。颗粒黄体细胞在单独的培养基中或在孕烯醇酮（10⁻⁷M）或睾酮（10⁻⁷M）存在的情况下培养2小时。颗粒黄体细胞中存在芳香化酶活性，睾酮存在时E2产量显著增加（P < 0.001）证明了这一点。添加孕烯醇酮并未增加Prog产量。II组患者来源的颗粒黄体细胞产生的Prog显著（P < 0.001）多于I组患者来源的颗粒黄体细胞。此外，与成熟卵母细胞-卵丘复合物相关的II组颗粒黄体细胞产生的Prog显著（P < 0.001）多于I组。受精和妊娠与Prog产量相关，即与受精卵母细胞相关的颗粒黄体细胞产生的Prog显著（P < 0.001）少于与未受精卵母细胞相关的颗粒黄体细胞。该系列中2名受孕患者的颗粒黄体细胞Prog产量进一步显著降低（P < 0.02）。看来在卵泡期早期（第1天）给予人绝经期促性腺激素会导致颗粒细胞成熟不完全。此外，就成功受精而言，最佳卵母细胞可能来自经过适当刺激导致充分成熟但未超过该阶段的卵泡。
