Prickaerts J P, Wilson G J, Bayliss C E, Baffour R
J Microsc. 1984 Aug;135(Pt 2):169-79. doi: 10.1111/j.1365-2818.1984.tb00517.x.
Morphometric determination of extracellular space in control and post-ischaemic reperfused rabbit myocardium was evaluated using two fixatives differing in their composition and total osmolality. Measurement of control extracellular space in an isotonic fixative (294 mOsm/kg water) was 20.8% and in a hypertonic fixative (1816 mOsm/kg water) was 22.2%. These values were not statistically different. Ischaemic durations of 15, 30, 60 and 90 min, followed by an equivalent period of reperfusion, created significant increases in extracellular space. The size of the extracellular space determined by both fixatives was found to be the same. Total fixative osmolality does not appear to influence morphometric evaluation of the extracellular space in control tissue or in tissue damaged by ischaemia and reperfusion.
使用两种成分和总渗透压不同的固定剂，对对照和缺血后再灌注的兔心肌细胞外间隙进行形态测定评估。在等渗固定剂（294 mOsm/kg水）中对照细胞外间隙的测量值为20.8%，在高渗固定剂（1816 mOsm/kg水）中为22.2%。这些值无统计学差异。缺血持续15、30、60和90分钟，随后进行相同时间的再灌注，会使细胞外间隙显著增加。发现两种固定剂所测定的细胞外间隙大小相同。总固定剂渗透压似乎不影响对照组织或因缺血和再灌注受损组织中细胞外间隙的形态测定评估。
