Mandl J, Tanács B, Spolarics Z, Garzó T, Mucha I, Antoni F, Machovich R, Horváth I
Thromb Res. 1984 Aug 15;35(4):407-14. doi: 10.1016/0049-3848(84)90232-9.
Uptake and metabolism of arachidonic acid, arachidic acid and oleic acid were investigated in isolated hepatocytes prepared from mouse liver with the collagenase perfusion method. The rate of uptake of arachidonic acid was time- and concentration- dependent. 94-98% of the arachidonic acid was incorporated into the phospholipid and triacylglycerol fractions following a 60 min incubation period at 37 degrees C. In the presence of thrombin-anti-thrombin III complex a change in the distribution of arachidonic acid incorporated into lipid fractions was found, i.e. increased incorporation into phosphatidyl-serine and phosphatidylethanolamine, whereas the uptake was not altered. There was no change in the uptake and incorporation of arachidic acid and oleic acid.
采用胶原酶灌注法从小鼠肝脏制备分离肝细胞，研究了花生四烯酸、花生酸和油酸的摄取及代谢。花生四烯酸的摄取速率具有时间和浓度依赖性。在37℃孵育60分钟后，94%-98%的花生四烯酸被掺入磷脂和三酰甘油组分中。在凝血酶-抗凝血酶III复合物存在的情况下，发现掺入脂质组分的花生四烯酸分布发生了变化，即掺入磷脂酰丝氨酸和磷脂酰乙醇胺的量增加，而摄取未改变。花生酸和油酸的摄取及掺入没有变化。
