使用阴离子交换树脂色谱法大规模纯化真菌糖化酶
Large-scale purification of fungal glucoamylases using anion-exchange resin chromatography.
作者信息
Bhella R S, Altosaar I
出版信息
Anal Biochem. 1984 Jul;140(1):200-2. doi: 10.1016/0003-2697(84)90154-4.
PMID:6435476
Abstract
Anion-exchange chromatography on polystyrene resin is shown to be more effective than DEAE-cellulose for purification of glucoamylase from crude enzyme extracts of Aspergillus awamori or from commercial preparations. The glucoamylase from A. awamori culture medium was purified to electrophoretic homogeneity with yields approaching 80%.
摘要
结果表明，在从泡盛曲霉的粗酶提取物或市售制剂中纯化糖化酶时，聚苯乙烯树脂阴离子交换色谱法比DEAE - 纤维素更有效。来自泡盛曲霉培养基的糖化酶被纯化至电泳纯，产率接近80%。
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