McIlroy P J, Bergert E R
Arch Biochem Biophys. 1984 Sep;233(2):652-60. doi: 10.1016/0003-9861(84)90491-0.
The ADP-ribosylation of rat luteal membrane proteins has been investigated. In the presence of cholera toxin two membrane proteins, Mr 115,000 and 46,000, incorporated [32P]ADP-ribose from [32P]NAD+. The larger protein also incorporated [32P]ADP-ribose in the absence of cholera toxin. The smaller protein was identified as the guanine nucleotide regulatory protein of adenylate cyclase. To facilitate studies concerning this species, a simple and convenient method of measuring ADP-ribose incorporation into the protein was developed. Levels of the protein were found to be approximately equal to those of the hCG receptor and 7- to 10-fold higher than those of the beta-adrenergic receptor. Luteinization of rat ovaries by injection of hCG indicated that G/F concentrations increased approximately 2-fold over a 5- to 11-day period, and correlated significantly with increased beta-adrenergic receptors, and beta-adrenergic and NaF-stimulated adenylate cyclase, but not with hCG receptors or hCG-stimulated adenylate cyclase. The distribution of the G/F protein in purified luteal membrane preparations mimicked the distribution of adenylate cyclase activity. No evidence could be found for hormonally induced alterations in ADP-ribose incorporation into this protein under either ribosylation or adenylate cyclase conditions. The exact role of the protein in the activation of rat luteal adenylate cyclase has yet to be determined.
对大鼠黄体膜蛋白的ADP-核糖基化进行了研究。在霍乱毒素存在的情况下，两种膜蛋白，分子量分别为115,000和46,000，从[32P]NAD+中掺入了[32P]ADP-核糖。较大的蛋白在没有霍乱毒素的情况下也掺入了[32P]ADP-核糖。较小的蛋白被鉴定为腺苷酸环化酶的鸟嘌呤核苷酸调节蛋白。为了便于对该蛋白进行研究，开发了一种简单便捷的测量ADP-核糖掺入该蛋白的方法。发现该蛋白的水平与hCG受体的水平大致相等，比β-肾上腺素能受体的水平高7至10倍。通过注射hCG使大鼠卵巢黄体化表明，G/F浓度在5至11天的时间内增加了约2倍，并且与β-肾上腺素能受体、β-肾上腺素能和NaF刺激的腺苷酸环化酶的增加显著相关，但与hCG受体或hCG刺激的腺苷酸环化酶无关。纯化的黄体膜制剂中G/F蛋白的分布与腺苷酸环化酶活性的分布相似。在核糖基化或腺苷酸环化酶条件下，均未发现激素诱导的该蛋白ADP-核糖掺入变化的证据。该蛋白在大鼠黄体腺苷酸环化酶激活中的具体作用尚待确定。
