嗜热四膜虫中的腐胺生物合成
Putrescine biosynthesis in Tetrahymena thermophila.
作者信息
Yao K M, Fong W F, Ng S F
出版信息
Biochem J. 1984 Sep 15;222(3):679-84. doi: 10.1042/bj2220679.
Abstract
The putrescine-biosynthesis pathway in Tetrahymena thermophila was delineated by studying crude extracts prepared from exponentially growing cultures. A pyridoxal phosphate-stimulated ornithine decarboxylase activity competitively inhibited by putrescine was detected. CO2 was also liberated from L-arginine, but analyses by t.l.c. and enzyme studies suggested that the activity was not due to arginine decarboxylase, nor could enzyme activities converting agmatine into putrescine be detected. We conclude that the decarboxylation of L-ornithine is probably the only major route for putrescine biosynthesis in this organism during exponential growth.
摘要
通过研究从指数生长期培养物中制备的粗提物，阐明了嗜热四膜虫中的腐胺生物合成途径。检测到一种受磷酸吡哆醛刺激、被腐胺竞争性抑制的鸟氨酸脱羧酶活性。L-精氨酸也会释放出CO₂，但薄层层析分析和酶学研究表明，该活性并非源于精氨酸脱羧酶，也未检测到将胍丁胺转化为腐胺的酶活性。我们得出结论，在指数生长期，L-鸟氨酸的脱羧作用可能是该生物体中腐胺生物合成的唯一主要途径。
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