Golub N B, Mevkh A T, Varfolomeev S D
Bioorg Khim. 1984 Feb;10(2):268-70.
The influence of some reagents modifying NH2-, SH-groups or imidazole moiety, on the prostaglandin endoperoxide synthetase activity was studied. Acetaldehyde, pyridoxal phosphate, dithiobis (nitrobenzoic) acid and iodoacetamide were found not to affect the enzyme activity. The activity was abolished as a result of the interaction with p-chloromercuribenzoic acid and diethyl pyrocarbonate. The hemin completely protected the apo-enzyme against the inactivation with diethyl pyrocarbonate. The assumption about the presence of imidazole moiety in the active site of the enzyme was made.
研究了一些修饰氨基、巯基或咪唑部分的试剂对前列腺素内过氧化物合成酶活性的影响。发现乙醛、磷酸吡哆醛、二硫代双（硝基苯甲酸）和碘乙酰胺不影响该酶的活性。与对氯汞苯甲酸和焦碳酸二乙酯相互作用导致该酶活性丧失。血红素完全保护脱辅基酶不被焦碳酸二乙酯灭活。由此推测该酶活性位点存在咪唑部分。
