Cho M I, Garant P R
Calcif Tissue Int. 1984 Jul;36(4):409-20. doi: 10.1007/BF02405353.
The effect of a proline analog, L-azetidine-2-carboxylic acid (LACA), on protein matrix secretion by odontoblasts and ameloblasts was compared by light and electron microscopic radioautography after injection of 3H-glycine in young mice. LACA inhibited the secretion of dentin matrix with consequent accumulation of 3H-glycine labeled procollagen in the cisternae of the rough endoplasmic reticulum. In contrast, LACA had no apparent effect on ameloblasts as enamel matrix continued to be packaged in the Golgi apparatus and secreted from Tomes' process within 30 min after injection of the radioprecursor. Electron microscopy revealed that LACA did not cause any change in ameloblast ultrastructure but produced a marked alteration of the odontoblast Golgi complex. All odontoblast Golgi saccules and collagen secretion granules disappeared within 2 h after LACA administration. Odontoblast Golgi cisternae, however, appeared not to be affected. These observations confirm previous studies conducted in this laboratory showing that Golgi saccules in collagen-secreting cells are the initial staging areas for the formation of secretory granules. These results also indicate that a close correlation exists between form and function in the Golgi apparatus of collagen-secreting cells.
在幼鼠体内注射³H-甘氨酸后，通过光学显微镜和电子显微镜放射自显影术，比较了脯氨酸类似物L-氮杂环丁烷-2-羧酸（LACA）对成牙本质细胞和成釉细胞分泌蛋白基质的影响。LACA抑制牙本质基质的分泌，导致³H-甘氨酸标记的前胶原在粗面内质网池内积聚。相比之下，LACA对成釉细胞没有明显影响，因为在注射放射性前体后30分钟内，釉质基质继续在高尔基体中包装，并从托姆斯突分泌出来。电子显微镜显示，LACA并未引起成釉细胞超微结构的任何变化，但导致成牙本质细胞高尔基体复合体发生明显改变。给予LACA后2小时内，所有成牙本质细胞的高尔基体囊泡和胶原分泌颗粒消失。然而，成牙本质细胞的高尔基体池似乎未受影响。这些观察结果证实了本实验室之前进行的研究，表明分泌胶原的细胞中的高尔基体囊泡是分泌颗粒形成的初始阶段区域。这些结果还表明，分泌胶原的细胞的高尔基体中形态与功能之间存在密切相关性。
