Bowman K A, Elijah R D, Cheeks K E, Green K
Curr Eye Res. 1984 Aug;3(8):991-1000. doi: 10.3109/02713688409011745.
Rabbit corneal endothelial pH and electrical potential have both been determined using tracer distribution techniques. Intercellular pH was measured using the dimethyloxazolidine-dione method and intracellular potential was measured using tetraphenylphosphonium bromide. Intracellular pH was determined as 7.10 in an ambient solution of pH 7.5. The only solution variations which altered intracellular pH were variations in the external solution pH, bathing in sodium-free or bicarbonate-free solution, incubation for 3 hours with 10(-6) or 10(-4) M ouabain or for 1 hour with 10(-4) M ouabain or in a high (60 mM) bicarbonate solution. The data indicate a close correlation between sodium and bicarbonate needs for the endothelium which corresponds with known effects of these ions on transendothelial ion fluxes. Intracellular potentials were measured of -34 mV, which were stable in the face of all environmental perturbations except 1 mM acetazolamide and 10(-6) M ouabain exposure for 3 hours. These newer techniques may be employed to provide some clues into the mechanism of endothelial transport systems.
兔角膜内皮细胞的pH值和电位均已通过示踪剂分布技术测定。细胞间pH值采用二甲基恶唑烷二酮法测量，细胞内电位采用溴化四苯基膦测量。在pH值为7.5的环境溶液中，细胞内pH值测定为7.10。唯一能改变细胞内pH值的溶液变化是外部溶液pH值的变化、在无钠或无碳酸氢盐溶液中浸泡、用10(-6)或10(-4) M哇巴因孵育3小时或用10(-4) M哇巴因孵育1小时或在高（60 mM）碳酸氢盐溶液中。数据表明内皮细胞对钠和碳酸氢盐的需求之间存在密切相关性，这与这些离子对跨内皮离子通量的已知影响相对应。测量到的细胞内电位为-34 mV，除了暴露于1 mM乙酰唑胺和10(-6) M哇巴因3小时外，在所有环境扰动下均保持稳定。这些更新的技术可用于为内皮运输系统的机制提供一些线索。
