大鼠子宫组织亚核组分中的雌二醇受体复合物
Oestradiol-receptor complexes in subnuclear fractions of rat uterine tissue.
作者信息
de Boer W, de Vries J, Mulder E, van der Molen H J
出版信息
Nucleic Acids Res. 1978 Jan;5(1):87-103. doi: 10.1093/nar/5.1.87.
The subnuclear distribution of 3H-oestradiol-receptor complexes was studied in uterine tissue of ovariectomized adult rats. Nuclei were sonically disrupted and 8 different subnuclear fractions were isolated by discontinuous sucrose density gradient centrifugation. 3H-Oestradiol-receptor complexes, measured by hydroxylapatite column chromatography, were localized in a light chromatin fraction as well as in a heavy chromatin fraction. Using the hydroxylapatite chromatography technique it was possible to demonstrate three classes of oestradiol-receptor complexes which differ in affinity for the chromatin. Oestradiol-receptor complexes with a high affinity for the chromatin were predominantly localized in the heavy chromatin fraction, whereas complexes with a lower affinity for their acceptor sites were present in the lighter chromatin fraction.
在成年去卵巢大鼠的子宫组织中研究了³H-雌二醇受体复合物的亚核分布。通过超声破碎细胞核，并通过不连续蔗糖密度梯度离心分离出8种不同的亚核组分。用羟基磷灰石柱色谱法测定的³H-雌二醇受体复合物定位于轻染色质组分和重染色质组分中。使用羟基磷灰石色谱技术可以证明三类雌二醇受体复合物，它们对染色质的亲和力不同。对染色质具有高亲和力的雌二醇受体复合物主要定位于重染色质组分中，而对其受体位点亲和力较低的复合物则存在于较轻的染色质组分中。
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