肝脏染色质的分级分离
Fractionation of liver chromatin.
作者信息
Marushige K, Bonner J
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2941-4. doi: 10.1073/pnas.68.12.2941.
Abstract
Rat liver chromatin has been fractionated into two fractions on the basis of precipitability in standard saline after mild treatment with DNase II. The major portion of liver chromatin contains little nonhistone protein and is enriched in histones, while a minor portion of such chromatin, with which RNA polymerase is associated, is highly enriched in proteins other than histone and impoverished in histones. Although the relative concentrations of the several histone species present in two chromatin fractions are identical, binding of histones to DNA in the minor fractions appears to be modified, presumably by the presence of proteins other than histones.
摘要
用脱氧核糖核酸酶II进行温和处理后，大鼠肝脏染色质已根据其在标准盐溶液中的沉淀性被分离成两个部分。肝脏染色质的主要部分几乎不含非组蛋白，富含组蛋白，而与RNA聚合酶相关的这部分染色质的一小部分则高度富含组蛋白以外的蛋白质，且组蛋白含量较低。尽管两个染色质部分中存在的几种组蛋白的相对浓度相同，但小部分中组蛋白与DNA的结合似乎发生了改变，推测是由于存在组蛋白以外的蛋白质。
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