具有β-半乳糖苷酶活性的杂合蛋白的一步纯化。
One-step purification of hybrid proteins which have beta-galactosidase activity.
作者信息
Ullmann A
出版信息
Gene. 1984 Jul-Aug;29(1-2):27-31. doi: 10.1016/0378-1119(84)90162-8.
PMID:6436145
Abstract
A one-step purification method of hybrid proteins exhibiting beta-galactosidase activity, based on affinity chromatography in the presence of high salt concentration, is described. Starting from crude bacterial extracts, several milligrams of near-homogeneous proteins can be obtained in a few hours with an overall yield of 85 to 95%. The purified hybrid proteins can be used to obtain antibodies against the foreign portion of the protein fusion.
摘要
本文描述了一种基于高盐浓度下亲和层析的、用于纯化具有β-半乳糖苷酶活性的杂合蛋白的一步法。从粗制细菌提取物开始，数小时内即可获得数毫克接近均一的蛋白质，总产率为85%至95%。纯化后的杂合蛋白可用于制备针对蛋白融合物中外源部分的抗体。
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