牛精浆中的核糖核酸酶与核糖核酸酶BS-1的一致性及其对聚硫酸乙烯酯的敏感性。

Identity of the ribonuclease from bovine seminal plasma with ribonuclease BS-1, and its sensitivity to polyvinyl sulphate.

作者信息

Ramakrishna T, Pandit M W

出版信息

J Biochem. 1984 Jul;96(1):253-60. doi: 10.1093/oxfordjournals.jbchem.a134820.

DOI:10.1093/oxfordjournals.jbchem.a134820

Abstract

A ribonuclease (RNAase BSD) isolated by us earlier from bovine seminal plasma by DNA-affinity chromatography is shown to be homogeneous on polyacrylamide gel electrophoresis, analytical ultracentrifuge and high performance liquid chromatography. From amino acid analysis, high performance liquid chromatography and enzymatic and physicochemical studies, this enzyme is shown to be identical to RNAase BS-1 reported by D'Alessio et al. ((1972) Eur. J. Biochem. 26, 153-161). Immunological studies support this observation. It has also been shown that, as compared to RNAase A, this enzyme is more sensitive to polyvinyl sulphate inhibition.

摘要

我们之前通过DNA亲和层析从牛精浆中分离出的一种核糖核酸酶（RNA酶BSD），在聚丙烯酰胺凝胶电泳、分析超速离心和高效液相色谱上显示为均一的。通过氨基酸分析、高效液相色谱以及酶学和物理化学研究表明，这种酶与D'Alessio等人（(1972) Eur. J. Biochem. 26, 153 - 161）报道的RNA酶BS - 1相同。免疫学研究支持这一观察结果。还表明，与RNA酶A相比，这种酶对聚硫酸乙烯酯抑制更敏感。