De Prisco R, Sorrentino S, Leone E, Libonati M
Biochim Biophys Acta. 1984 Aug 14;788(3):356-63. doi: 10.1016/0167-4838(84)90049-9.
A ribonuclease, active on single- and double-stranded RNAs, has been isolated from human seminal plasma 3-5 micrograms of enzyme were recovered per ml of seminal plasma, equivalent to 71% of total activity and a 2500-fold purification (measured with poly(A) X poly(U) as substrate) from the initial dialyzed material. Similar amounts of RNAase were found per g (wet weight) of human prostate, where the enzyme appears to be produced. Human seminal RNAase degrades poly(U) 3-times faster than poly(A) X poly(U), and poly(C) or viral single-stranded RNA about 10-times faster than poly(U). Degradation of poly(A) X poly(U), viral double-stranded RNA, and poly(A) by human seminal RNAase is 500-, 380- and 140-times more efficient, respectively, than by bovine RNAase A. The enzyme, a basic protein with maximum absorbance at 276 nm, occurs in two almost equivalent forms, one of which is glycosylated. Mr values of the glycosylated and non-glycosylated form are 21000 and 16000, respectively. The amino-acid composition of the RNAase is very similar to that of human pancreatic RNAase. The same is true for the carbohydrate content of its glycosylated form.
已从人精浆中分离出一种对单链和双链RNA均有活性的核糖核酸酶。每毫升精浆可回收3 - 5微克该酶，相当于总活性的71%，且相对于初始透析材料有2500倍的纯化效果（以聚（A）×聚（U）为底物测定）。在每克（湿重）人前列腺中发现了类似量的RNA酶，该酶似乎就是在前列腺中产生的。人精浆RNA酶降解聚（U）的速度比聚（A）×聚（U）快3倍，降解聚（C）或病毒单链RNA的速度比聚（U）快约10倍。人精浆RNA酶对聚（A）×聚（U）、病毒双链RNA和聚（A）的降解效率分别比牛RNA酶A高500倍、380倍和140倍。该酶是一种碱性蛋白质，在276nm处有最大吸光度，以两种几乎等量的形式存在，其中一种是糖基化的。糖基化形式和非糖基化形式的相对分子质量分别为21000和16000。该RNA酶的氨基酸组成与人胰腺RNA酶非常相似。其糖基化形式的碳水化合物含量也是如此。
