Mukai R, Suzuki M, Yabe T, Hamaguchi H, Maeda H
J Immunol. 1984 Dec;133(6):3211-9.
Human B lymphoblastoid cell lines are known to express several class II antigens. The MT3 specificity defined by alloantisera is in linkage disequilibrium with DR4, DR7, and DRw9, and is called a "supertypic" specificity. To identify the MT3 molecule, immunoprecipitates of HLA-DR and MT3 antigens from extracts of three metabolically labeled HLA-DR homozygous cell lines (Wa;DR4, L-Pi-tot;DR7, L-KT12;DRw9) were analyzed by two-dimensional gel electrophoresis. The light chains of the MT3 antigens from DR4, DR7, and DRw9 homozygous cell lines were indistinguishable from one another in charge and m.w. The light chains of the MT3 antigens differed from those of DR4, DR7, and DRw9 antigens, whereas the heavy chains of the MT3 and DR antigens were indistinguishable. The MT3 antigen appears to share the same heavy chain as the DR antigens. However, this antigen would constitute a new human class II molecule distinct from the DR antigen. These data also raised the possibility that the light chains of the MT3 molecules may be the products of a new locus different from the DR locus.
已知人类B淋巴母细胞系表达多种Ⅱ类抗原。同种异体抗血清所定义的MT3特异性与DR4、DR7和DRw9处于连锁不平衡状态，被称为“超型”特异性。为鉴定MT3分子，通过二维凝胶电泳分析了来自三种经代谢标记的HLA - DR纯合细胞系（Wa；DR4，L - Pi - tot；DR7，L - KT12；DRw9）提取物中的HLA - DR和MT3抗原的免疫沉淀物。DR4、DR7和DRw9纯合细胞系的MT3抗原轻链在电荷和分子量方面彼此无法区分。MT3抗原的轻链与DR4、DR7和DRw9抗原的轻链不同，而MT3和DR抗原的重链无法区分。MT3抗原似乎与DR抗原共享相同的重链。然而，该抗原将构成一种不同于DR抗原的新的人类Ⅱ类分子。这些数据还提出了一种可能性，即MT3分子的轻链可能是不同于DR基因座的新基因座的产物。
