Kramer T R, Briske-Anderson M, Johnson S B, Holman R T
J Nutr. 1984 Nov;114(11):2047-52. doi: 10.1093/jn/114.11.2047.
The effects of biotin and protein-calorie deficiency on metabolism of liver phospholipids in rats were determined. Weanling male Long-Evans rats were fed ad libitum a biotin-deficient (0.4 micrograms biotin/g diet) or a biotin-adequate (2.4 micrograms biotin/g diet) diet for 49 days. A pair-fed group was fed biotin-adequate diet but restricted to the amount consumed by biotin-deficient rats. The effects of these diets on food intake, weight gain, biotin content of serum and fatty acid composition of liver phospholipid were determined. Growth and food intake were significantly depressed in biotin-deficient and pair-fed rats. Biotin concentration in serum was significantly reduced in biotin-deficient and in pair-fed rats. Biotin deficiency caused significantly increased concentrations of 15:0, 17:0 and total odd-chain fatty acids in liver phospholipids. Combined biotin and protein-calorie deficiency and protein-calorie deficiency alone caused significantly increased concentrations of 18:2 omega 6 in liver phospholipids. Biotin deficiency caused significant reduction in liver phospholipid 20:3 omega 6. Reduced arachidonic acid (20:4 omega 6) level was due to combined biotin protein-calorie deficiency. Protein-calorie deficiency alone was primarily responsible for reduced levels of 22:5 omega 6. Biotin deficiency alone caused significant increases in liver phospholipids 18:3 omega 3 and 22:6 omega 3.
测定了生物素和蛋白质-热量缺乏对大鼠肝脏磷脂代谢的影响。将断乳雄性Long-Evans大鼠随意喂食生物素缺乏（0.4微克生物素/克日粮）或生物素充足（2.4微克生物素/克日粮）的日粮49天。一对对照喂养组喂食生物素充足的日粮，但摄入量限制为生物素缺乏大鼠的摄入量。测定了这些日粮对食物摄入量、体重增加、血清生物素含量和肝脏磷脂脂肪酸组成的影响。生物素缺乏和对照喂养的大鼠的生长和食物摄入量显著降低。生物素缺乏和对照喂养的大鼠血清中的生物素浓度显著降低。生物素缺乏导致肝脏磷脂中15:0、17:0和总奇数链脂肪酸的浓度显著增加。生物素和蛋白质-热量联合缺乏以及单独的蛋白质-热量缺乏导致肝脏磷脂中18:2ω6的浓度显著增加。生物素缺乏导致肝脏磷脂20:3ω6显著减少。花生四烯酸（20:4ω6）水平降低是由于生物素与蛋白质-热量联合缺乏所致。单独的蛋白质-热量缺乏是导致22:5ω6水平降低的主要原因。单独的生物素缺乏导致肝脏磷脂18:3ω3和22:6ω3显著增加。
