Costlow N, Lis J T
Mol Cell Biol. 1984 Sep;4(9):1853-63. doi: 10.1128/mcb.4.9.1853-1863.1984.
High-resolution analysis of the chromatin structure of the promoter regions of five Drosophila heat shock genes showed a similar location for the hypersensitive sequences relative to the start of transcription. For each of the five genes examined--those coding for hsp27, hsp26, hsp23, hsp70, and hsp83--the DNase I-hypersensitive sites in Drosophila melanogaster nuclei mapped to two regions upstream of the coding region. These sites occurred on the average, 115 and 17 base pairs upstream from the start of transcription of the five heat shock genes examined. This latter site corresponded to sequences at or near the TATA consensus sequence. Sites even further upstream of the hsp27, hsp26, and hsp83 genes were also evident. Additionally, for the two genes examined--hsp70 and hsp83--the DNase I-hypersensitive sites were preserved, at least within this level of resolution (+/- 10 base pairs), when the Drosophila genes were integrated into the Saccharomyces cerevisiae genome. This result indicates that the signals responsible for generating these hypersensitive sites are inherent in the DNA sequences and, in this case, are not highly species specific.
对五个果蝇热休克基因启动子区域的染色质结构进行的高分辨率分析表明，超敏序列相对于转录起始点的位置相似。对于所检测的五个基因中的每一个——即编码hsp27、hsp26、hsp23、hsp70和hsp83的基因——黑腹果蝇细胞核中的DNA酶I超敏位点定位于编码区域上游的两个区域。这些位点平均出现在所检测的五个热休克基因转录起始点上游115和17个碱基对处。后一个位点对应于TATA共有序列处或其附近的序列。hsp27、hsp26和hsp83基因更上游的位点也很明显。此外，对于所检测的两个基因——hsp70和hsp83——当果蝇基因整合到酿酒酵母基因组中时，DNA酶I超敏位点得以保留，至少在这种分辨率水平（±10个碱基对）内。这一结果表明，产生这些超敏位点的信号是DNA序列所固有的，在这种情况下，并非高度物种特异性的。
