Daimon M, Tsutsumi K, Sato J, Tsutsumi R, Ishikawa K
Biochem Biophys Res Commun. 1984 Oct 30;124(2):337-43. doi: 10.1016/0006-291x(84)91558-4.
The expression of aldolase A and B mRNAs during azo-dye-induced carcinogenesis in rat liver was examined. After feeding the dye for 18 weeks, the level of aldolase A mRNA increased to about 11 times that in a normal liver, with the concomitant decrease of aldolase B mRNA level to about 25% of that in a normal liver. These changes did not occur progressively during the carcinogenesis, but occurred as an additional phase after 4 week-feeding of the azo-dye. At this stage, the levels of aldolase A and B mRNAs were about 7 times and 45% of that in a normal liver, respectively. This biphasic pattern in the aldolase isozyme expression in the azo-dye-fed rat liver is discussed together with the kinetic data of the enzyme activity.
研究了偶氮染料诱导大鼠肝脏癌变过程中醛缩酶A和B mRNA的表达情况。在给大鼠喂食该染料18周后，醛缩酶A mRNA水平增加至正常肝脏的约11倍，同时醛缩酶B mRNA水平降至正常肝脏的约25%。这些变化在癌变过程中并非渐进发生，而是在喂食偶氮染料4周后作为一个附加阶段出现。在此阶段，醛缩酶A和B mRNA水平分别约为正常肝脏的7倍和45%。结合酶活性的动力学数据，讨论了喂食偶氮染料的大鼠肝脏中醛缩酶同工酶表达的这种双相模式。
