Mangalo R, Marcovich H
C R Acad Sci III. 1984;299(11):445-50.
Fetal calf serum (SVF), insolubilized on a sepharose matrix, retains mitogenic factors (FM) from horse serum (SC) which can be eluted by molar acetic acid. The FM can: 1 degree initiate the synthesis of DNA in serum starved, contact inhibited 3T3 BALB/c fibroblasts and 2 degrees allow at least three generations to take place. The FM have no antitryptic activity and do not allow cell attachment. Weight to weight, this fraction is 10 to 50 times more active than horse serum. Polyacrylamide gel electrophoresis shows that various proteins are present, particularly immunoglobulins. The intervention of epidermal growth factor (EGF), fibroblast growth factor (FGF) or the platelet-derived growth factor (PDGF) have been excluded.
固定在琼脂糖基质上的胎牛血清（SVF）保留了来自马血清（SC）的促有丝分裂因子（FM），该因子可用摩尔乙酸洗脱。FM能够：1）启动血清饥饿、接触抑制的3T3 BALB/c成纤维细胞中的DNA合成；2）使细胞至少传代三代。FM没有抗胰蛋白酶活性，也不允许细胞附着。按重量计算，该组分的活性比马血清高10至50倍。聚丙烯酰胺凝胶电泳显示存在多种蛋白质，尤其是免疫球蛋白。已排除表皮生长因子（EGF）、成纤维细胞生长因子（FGF）或血小板衍生生长因子（PDGF）的作用。
