Murphy W A, Lumsden R D
J Parasitol. 1984 Aug;70(4):516-21.
Using nonpermeating, radiolabeled solutes to estimate the magnitude of the "unstirred water layer" (="mucosal epithelial space") of the surface of Hymenolepis diminuta, a value approximating 1% of the worm's fluid volume (0.011-0.022 ml/g wet tissue) was obtained. This value was compared with those previously reported by other workers which were greater by an order of magnitude. The difference between these results appears to be related to the use in previous studies of a permeating marker (mannitol), and a failure to divest the surface of nonspecifically adherent bathing fluid in excess of the actual "unstirred layer". These parameters must be considered in future studies on this useful model for the study of transport.
利用非渗透性放射性标记溶质来估算微小膜壳绦虫体表“未搅动水层”(即“黏膜上皮间隙”)的大小,得到了一个约为虫体液体体积1%(0.011 - 0.022毫升/克湿组织)的值。该值与其他研究者先前报道的值进行了比较,他们的值要大一个数量级。这些结果之间的差异似乎与先前研究中使用渗透性标记物(甘露醇)以及未能去除超过实际“未搅动层”的非特异性附着的浴液表面有关。在今后关于这个用于研究转运的有用模型的研究中,必须考虑这些参数。