Inhibition of the chicken retinal UDP-GaINAc:GM3, N-acetylgalactosaminyl-transferase by blood serum and by pineal gland extracts.

The effects of the pineal gland extract and blood serum on the activity of the UDP-GalNAc:GM3, N-acetylgalactosaminyltransferase (GM3:GalNAc-T) from chicken retina were studied. Both preparations have inhibitory capability on the enzyme activity. Two types of inhibitory capabilities were found: one is heat labile and decomposes UDP-GalNAc and another is heat stable. When the pineal gland extracts were prepared from light-exposed chickens, the inhibitory capability increased with respect to the extracts from dark-maintained animals; vice versa, blood serum from dark-maintained animals had higher heat-labile and heat-stable capabilities than that from light-exposed chickens. In in vitro experiments, no difference was found in the inhibitory capability of blood serum extracts from pinealectomized animals compared to control animals. In vivo labeling experiments with pinealectomized animals in either light or dark showed similar differences in the labeling of the optic tectum gangliosides as the normal animals.