Targan S, Decker J M, Ades E W
Nat Immun Cell Growth Regul. 1983;3(3):113-23.
The mechanism of lysis by cytotoxic T lymphocytes, K cells, and natural killer (NK) cells is imperfectly understood at this point. In this report, material (glycopeptide) isolated from the plasma membranes of K562 cells and fractionated on lectin affinity adsorbents which has been shown to inhibit NK lysis, was used in several specific NK assays to ascertain what stages of the NK-lytic sequence is inhibited by this substance. Results indicate that this glycopeptide (a) does not inhibit initial binding, but dissociates conjugates following initial effector target interactions; (b) inhibits NK lysis beyond Ca-dependent programming, and (c) inhibits lysis induced by NK cell-derived soluble cytotoxic factors (NKCF) in a soluble factor assay. These results suggest that this glycopeptide can effect the lethal hit stage of NK lysis and may represent structures which can associate directly with NKCF.
目前，细胞毒性T淋巴细胞、K细胞和自然杀伤（NK）细胞的裂解机制尚未完全明确。在本报告中，从K562细胞膜中分离并在凝集素亲和吸附剂上分级分离得到的物质（糖肽）已被证明可抑制NK裂解，该物质被用于多种特异性NK检测中，以确定该物质抑制NK裂解序列的哪些阶段。结果表明，这种糖肽（a）不抑制初始结合，但在初始效应细胞与靶细胞相互作用后使结合物解离；（b）在依赖钙的编程之后抑制NK裂解，并且（c）在可溶性因子检测中抑制由NK细胞衍生的可溶性细胞毒性因子（NKCF）诱导的裂解。这些结果表明，这种糖肽可以影响NK裂解的致命打击阶段，并且可能代表可以直接与NKCF结合的结构。
