Wood W I, Capon D J, Simonsen C C, Eaton D L, Gitschier J, Keyt B, Seeburg P H, Smith D H, Hollingshead P, Wion K L, Delwart E, Tuddenham E G, Vehar G A, Lawn R M
Nature. 1984;312(5992):330-7. doi: 10.1038/312330a0.
DNA clones encoding the complete 2,351 amino acid sequence for human factor VIII have been isolated and used to produce biologically active factor VIII in cultured mammalian cells. The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and immunological characteristics of serum-derived factor VIII.
已分离出编码完整2351个氨基酸序列的人凝血因子VIII的DNA克隆，并用于在培养的哺乳动物细胞中生产具有生物活性的凝血因子VIII。该重组蛋白可纠正血友病患者血浆的凝血时间，并具有许多血清来源的凝血因子VIII的生化和免疫特性。
