Takagi M
Acta Pathol Jpn. 1984 Sep;34(5):1099-114. doi: 10.1111/j.1440-1827.1984.tb07639.x.
Ultrastructural and immunohistochemical studies of six cardiac myxomas were carried out to further elucidate the histogenesis of this tumor and the cell character of myxoma cell. Ultrastructurally myxoma cells have varying degrees of endothelial characteristics with prominent vasoformative activity. Immunohistochemically, blood group H-antigen and Factor VIII-related antigen were identified in tumor cells of all six cases. Prostaglandin I2 was demonstrated in five. The contractile proteins (actin and myosin), basement membrane components (laminin and fibronectin) were identified in all cases. Intermediate filament vimentin was demonstrated in two cases. On the other hand, desmin, keratin, and glial fibrillary acidic protein were not demonstrated. This result further supports the concept of the endothelial origin of cardiac myxoma rather than origin form multipotential mesenchymal cells. Myxoma cells infiltrated and covered the fibrin thrombi attached to the surface of the tumor, and the fibrin thrombi were replaced by myxoid matrix as the myxoma cells infiltrated it. This finding suggests that fibrin thrombi may be related to the growth of cardiac myxoma and active synthesis of prostaglandin I2 in myxoma cells may be due to intimate contact with platelet-rich fibrin thrombi attached to the tumor.
对6例心脏黏液瘤进行了超微结构和免疫组织化学研究，以进一步阐明该肿瘤的组织发生及黏液瘤细胞的细胞特征。超微结构上，黏液瘤细胞具有不同程度的内皮细胞特征，血管形成活性显著。免疫组织化学方面，6例肿瘤细胞中均检测出血型H抗原和Ⅷ因子相关抗原。5例检测到前列腺素I2。所有病例均检测到收缩蛋白（肌动蛋白和肌球蛋白）及基底膜成分（层粘连蛋白和纤连蛋白）。2例检测到中间丝波形蛋白。另一方面，未检测到结蛋白、角蛋白和胶质纤维酸性蛋白。这一结果进一步支持心脏黏液瘤起源于内皮细胞而非多能间充质细胞的观点。黏液瘤细胞浸润并覆盖附着于肿瘤表面的纤维蛋白血栓，随着黏液瘤细胞浸润，纤维蛋白血栓被黏液样基质取代。这一发现提示纤维蛋白血栓可能与心脏黏液瘤的生长有关，黏液瘤细胞中前列腺素I2的活跃合成可能是由于与附着于肿瘤的富含血小板的纤维蛋白血栓密切接触所致。
