Bizec J C, Klethi J, Mandel P
C R Acad Sci III. 1984;299(8):267-70.
Calmodulin contents were determined by radioimmunoassay in Bovine lens layers as a function of age. The values in the epithelial cell layer appeared to be strikingly higher than in the cortical layer where they were still higher than in the nucleus. In the lens epithelial cell layer the calmodulin levels were maintained when calf was compared with adult or aged animals (100-110 ng/mg protein). Nevertheless, a decrease of calmodulin content was observed in the lens epithelium of very old animals (65 ng/mg prot.). In the cortical layer, the calmodulin content was very low and variable during aging (11-18 ng/mg prot.). In the lens nucleus the extremely low amount of calmodulin still decreased during aging (10-0.2 ng/mg prot.). The significance of these data was discussed.
采用放射免疫分析法测定了不同年龄牛晶状体各层中的钙调蛋白含量。上皮细胞层中的钙调蛋白含量明显高于皮质层，而皮质层中的含量又高于核层。在晶状体上皮细胞层，将小牛与成年或老年动物相比，钙调蛋白水平保持稳定（100 - 110 ng/mg蛋白质）。然而，在非常老龄动物的晶状体上皮中观察到钙调蛋白含量下降（65 ng/mg蛋白质）。在皮质层，钙调蛋白含量在衰老过程中非常低且变化不定（11 - 18 ng/mg蛋白质）。在晶状体核层，极低含量的钙调蛋白在衰老过程中仍会下降（10 - 0.2 ng/mg蛋白质）。文中讨论了这些数据的意义。
