Kudrjashov B A, Liapina L A, Klöcking H P
Folia Haematol Int Mag Klin Morphol Blutforsch. 1976;103(4):573-82.
The obtained heparin-ocrase complex includes properties of inhibiting coagulation and a high fibrinolytic activity in vitro and in vivo. The fibrinolytic activity of the heparin-ocrase complex is higher than the equivalent amount of ocrase. The fibrinolytic activity of the complex is not only evident on non-stabilized fibrin plates, but likewise on stabilized ones and at the presence of inhibitors of enzymatic fibrinolysis, such as Trasylol and epsilon-aminocaproic acid. The complex formation between heparin and protease was confirmed by means of cross-paper electrophoresis and spectrophotometry.
所获得的肝素-奥克酶复合物具有体外和体内抑制凝血的特性以及高纤溶活性。肝素-奥克酶复合物的纤溶活性高于等量的奥克酶。该复合物的纤溶活性不仅在未稳定的纤维蛋白平板上明显,在稳定的平板上以及存在酶促纤溶抑制剂(如抑肽酶和ε-氨基己酸)的情况下同样明显。通过交叉纸电泳和分光光度法证实了肝素与蛋白酶之间形成了复合物。