Buessow S C, Gillespie G Y
J Biol Response Mod. 1984 Dec;3(6):653-62.
Two different preparations of ultrapurified interferon-alpha (IFN-alpha) (lymphoblastoid and peripheral blood leukocyte) and one of IFN-gamma were tested for their ability to induce terminal differentiation and alter cell growth in three human leukemia cell lines of different hematological origin (HL-60, K562, U937). Cell lines were cultured for 9 days in the presence of 500 units/ml of either IFN-alpha or of IFN-gamma. Cell counts and stained differentials were made on days 3, 6, and 9 to assess the effects of IFN. A marked heterogeneity of response was found, not only among cell lines, but among the IFNs tested. The most striking morphological changes were noted in the HL-60 acute promyelocytic leukemia cell line. All three IFNs tested induced significant myeloid maturation, with increased numbers of terminally differentiated myelocytes and metamyelocytes seen as early as day 3 of culture. The feasibility of using IFNs as an adjunctive or alternative therapy for the treatment of some types of leukemias is discussed.
对两种不同的超纯化α干扰素制剂（淋巴母细胞样和外周血白细胞来源）以及一种γ干扰素，检测了它们在三种不同血液学来源的人类白血病细胞系（HL-60、K562、U937）中诱导终末分化和改变细胞生长的能力。细胞系在500单位/毫升的α干扰素或γ干扰素存在下培养9天。在第3、6和9天进行细胞计数和染色分类，以评估干扰素的作用。发现不仅在细胞系之间，而且在所测试的干扰素之间，反应存在明显的异质性。在HL-60急性早幼粒细胞白血病细胞系中观察到最显著的形态学变化。所测试的所有三种干扰素均诱导了显著的髓系成熟，早在培养第3天就可见终末分化的髓细胞和晚幼粒细胞数量增加。文中讨论了将干扰素用作某些类型白血病辅助治疗或替代治疗的可行性。
