Stuart K, Gobright E, Jenni L, Milhausen M, Thomashow L, Agabian N
J Parasitol. 1984 Oct;70(5):747-54.
An extensive serodeme of sequentially-isolated antigenic variants of African trypanosomes has been produced from both syringe-passaged and cyclically-transmitted Trypanosoma brucei of the IsTaR 1 clone derived from EATRO 164. The majority of the antigenic variants were isolated from chronically-infected deer mice (Peromyscus leucopus). The pattern of parasitemias during the course of infections initiated with syringe-passaged trypanosomes differed from those initiated with cyclically-transmitted trypanosomes. Trypanosome populations from syringe-passaged (192) and cyclically-transmitted (31) clones were each amplified by growth in lethally-irradiated mice and cryopreserved for retrospective analysis. Five clones derived from a single deer mouse during the first 44 days of infection, and 2 clones derived from an acutely-infected rat were established from these amplified populations. Homogeneous populations were grown in lethally-irradiated rats and mice for antigenic analysis purification of variant-specific glycoprotein. Six of the 7 clones were distinct variants by immunological criteria using antisera derived from whole cells or purified surface glycoproteins. Two clones, one derived from the acutely-infected rat, and the other from the first parasitemia in a chronic infection that was initiated with the former clone, were immunologically identical. Production of these clones established a well-defined serodeme that will allow detailed analysis of antigenic variation.
从源自EATRO 164的IsTaR 1克隆的经注射器传代和周期性传播的布氏锥虫中，已产生了广泛的非洲锥虫序列分离抗原变异体血清型。大多数抗原变异体是从慢性感染的鹿鼠（白足鼠）中分离出来的。由经注射器传代的锥虫引发的感染过程中的寄生虫血症模式与由周期性传播的锥虫引发的感染过程中的模式不同。来自经注射器传代（192个）和周期性传播（31个）克隆的锥虫群体分别通过在经致死性照射的小鼠中生长进行扩增，并冷冻保存以供回顾性分析。从这些扩增群体中建立了在感染的前44天内从一只鹿鼠中获得的5个克隆，以及从一只急性感染大鼠中获得的2个克隆。在经致死性照射的大鼠和小鼠中培养均匀群体，用于抗原分析和变异体特异性糖蛋白的纯化。使用源自全细胞或纯化表面糖蛋白的抗血清，根据免疫学标准，7个克隆中的6个是不同的变异体。两个克隆，一个源自急性感染的大鼠，另一个源自由前一个克隆引发的慢性感染中的首次寄生虫血症，在免疫学上是相同的。这些克隆的产生建立了一个定义明确的血清型，这将允许对抗原变异进行详细分析。
