• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在对血栓素合成酶进行药理阻断后，采用放射免疫分析法和高分辨率气相色谱 - 质谱联用技术定量测定血清血栓素B2和6 - 酮 - 前列环素F1α的比较

Comparison of radioimmunoassay and high-resolution gas chromatography mass spectrometry for the quantitative determination of serum thromboxane B2 and 6-keto-PGF1 alpha after pharmacological blockade of thromboxane synthetase.

作者信息

Chiabrando C, Castagnoli M N, Noseda A, Fanelli R, Rajtar G, Cerletti C, de Gaetano G

出版信息

Prostaglandins Leukot Med. 1984 Oct;16(1):79-88. doi: 10.1016/0262-1746(84)90088-x.

DOI:10.1016/0262-1746(84)90088-x
PMID:6440153
Abstract

Radioimmunoassay (RIA) and high-resolution gas chromatography-mass spectrometry (HRGC-MS) were compared for the determination of serum 6-keto-PGF-1 alpha and TXB2 in a situation of drug-altered arachidonate metabolism. Results were comparable for TXB2 in both conditions. 6-keto-PGF1 alpha RIA determinations (with two different antisera) revealed increased levels in dazoxiben-treated samples, which were not confirmed by HRGC-MS. The assay were repeatedly checked in controlled conditions to investigate these discrepancies. Interference was found with both antisera, due to the drug-induced change in metabolism.

摘要

在药物改变花生四烯酸代谢的情况下，比较了放射免疫分析法（RIA）和高分辨率气相色谱-质谱联用技术（HRGC-MS）用于测定血清6-酮-前列腺素F-1α和血栓素B2的效果。两种情况下血栓素B2的结果具有可比性。6-酮-前列腺素F-1α的放射免疫分析测定（使用两种不同抗血清）显示，用达唑氧苯处理的样本中该物质水平升高，但高分辨率气相色谱-质谱联用技术未证实这一结果。在对照条件下对该检测方法进行了反复检查以研究这些差异。发现两种抗血清均存在干扰，这是由药物诱导的代谢变化所致。

相似文献

1
Comparison of radioimmunoassay and high-resolution gas chromatography mass spectrometry for the quantitative determination of serum thromboxane B2 and 6-keto-PGF1 alpha after pharmacological blockade of thromboxane synthetase.在对血栓素合成酶进行药理阻断后，采用放射免疫分析法和高分辨率气相色谱 - 质谱联用技术定量测定血清血栓素B2和6 - 酮 - 前列环素F1α的比较
Prostaglandins Leukot Med. 1984 Oct;16(1):79-88. doi: 10.1016/0262-1746(84)90088-x.
2
In vivo redirection of prostaglandin endoperoxides into 6-keto PGF1 alpha formation by thromboxane synthetase inhibitors in the rat.在大鼠体内，血栓素合成酶抑制剂将前列腺素内过氧化物重定向为6-酮-前列腺素F1α的生成。
Thromb Res. 1983 Oct 1;32(1):15-27. doi: 10.1016/0049-3848(83)90150-0.
3
A comparison of human pulmonary arterial and venous prostacyclin and thromboxane synthesis--effect of a thromboxane synthase inhibitor.人肺动脉和静脉前列环素与血栓素合成的比较——血栓素合酶抑制剂的作用
Thromb Haemost. 1984 Apr 30;51(2):257-60.
4
Effects of a thromboxane synthetase inhibitor and a thromboxane antagonist on release and activity of thromboxane A2 and prostacyclin in vitro.血栓素合成酶抑制剂和血栓素拮抗剂对体外血栓素A2和前列环素释放及活性的影响。
Prostaglandins. 1985 May;29(5):785-97. doi: 10.1016/0090-6980(85)90138-8.
5
Production of thromboxane and prostaglandins in human blood in the presence of thromboxane synthase inhibitors: a comparison of RIA and GC/MS determinations.血栓素合酶抑制剂存在下人体血液中血栓素和前列腺素的生成：放射免疫分析与气相色谱/质谱测定法的比较
Blood Coagul Fibrinolysis. 1991 Feb;2(1):7-15. doi: 10.1097/00001721-199102000-00002.
6
Prostaglandin and thromboxane synthesis by M5076 ovarian reticulosarcoma during growth: effects of a thromboxane synthetase inhibitor.M5076卵巢网织肉瘤生长过程中前列腺素和血栓素的合成：血栓素合成酶抑制剂的作用
Cancer Res. 1987 Feb 15;47(4):988-91.
7
Intravenous infusion of a selective inhibitor of thromboxane A2 synthetase in man: influence on thromboxane B2 and 6-keto-prostaglandin F1 alpha levels and platelet aggregation.人静脉输注血栓素A2合成酶选择性抑制剂：对血栓素B2和6-酮-前列腺素F1α水平及血小板聚集的影响
Circulation. 1984 Oct;70(4):599-605. doi: 10.1161/01.cir.70.4.599.
8
The effect of a thromboxane synthetase inhibitor, dazoxiben, and acetylsalicylic acid on platelet function and prostaglandin metabolism.血栓素合成酶抑制剂达唑氧苯和乙酰水杨酸对血小板功能及前列腺素代谢的影响。
Thromb Haemost. 1983 Oct 31;50(3):703-6.
9
Quantitative profiling of 6-ketoprostaglandin F1 alpha, 2,3-dinor-6-ketoprostaglandin F1 alpha, thromboxane B2 and 2,3-dinor-thromboxane B2 in human and rat urine by immunoaffinity extraction with gas chromatography-mass spectrometry.采用免疫亲和萃取结合气相色谱-质谱法对人和大鼠尿液中的6-酮前列环素F1α、2,3-二去甲-6-酮前列环素F1α、血栓素B2和2,3-二去甲-血栓素B2进行定量分析。
J Chromatogr. 1989 Oct 27;495:1-11. doi: 10.1016/s0378-4347(00)82604-3.
10
Inhibition of thromboxane biosynthesis in serum: limitations of the measurement of immunoreactive 6-keto-PGF1 alpha.血清中血栓素生物合成的抑制：免疫反应性6-酮-前列腺素F1α测量的局限性
Thromb Res. 1984 Jan 1;33(1):99-103. doi: 10.1016/0049-3848(84)90159-2.