López-Botet M, Toribio M L, Moretta L, De Landázuri M O
Eur J Immunol. 1984 Dec;14(12):1137-41. doi: 10.1002/eji.1830141214.
The role of interleukin 2 (IL 2) and interferon (IFN)-gamma in the maintenance of natural killer (NK)-like activity mediated by human cytolytic T cell clones was investigated. When cultured in standard supernatant (SN) obtained by phytohemagglutinin stimulation of human mononuclear cells (containing both IL2 and IFN activities), several mixed lymphocyte culture-derived clones displayed cytolytic activity directed against the K562 cell line; in addition, some of these clones also lysed specific target cells bearing the sensitizing alloantigens. When cultured in IL2-containing SN obtained from the Jurkat cell line, the NK-like activity was selectively abrogated within 4-5 days. However, by culturing cells again in standard SN the cytolytic activity was completely restored. The possibility that inhibitory molecules in Jurkat SN could be responsible for the loss of NK-like activity was ruled out by experiments showing that cytotoxicity could be induced by mixtures of Jurkat and standard SN. In addition, a source of affinity chromatography-purified IL2 also failed to maintain NK-like activity. Moreover, addition of recombinant IFN-gamma in combination with purified IL2 did not prevent the loss of NK-like activity. The present results indicate that neither IL2 nor IFN-gamma are sufficient to maintain NK-like activity and suggest that other molecule(s) are required.
研究了白细胞介素2（IL-2）和干扰素（IFN）-γ在维持人细胞毒性T细胞克隆介导的自然杀伤（NK）样活性中的作用。当在通过植物血凝素刺激人单核细胞获得的标准上清液（SN）（含有IL-2和IFN活性）中培养时，几个混合淋巴细胞培养衍生的克隆表现出针对K562细胞系的细胞溶解活性；此外，这些克隆中的一些还裂解携带致敏同种异体抗原的特异性靶细胞。当在从Jurkat细胞系获得的含IL-2的SN中培养时，NK样活性在4-5天内被选择性地消除。然而，通过再次在标准SN中培养细胞，细胞溶解活性完全恢复。通过实验排除了Jurkat SN中的抑制分子可能导致NK样活性丧失的可能性，该实验表明Jurkat和标准SN的混合物可诱导细胞毒性。此外，亲和层析纯化的IL-2来源也未能维持NK样活性。此外，重组IFN-γ与纯化的IL-2联合添加并不能阻止NK样活性的丧失。目前的结果表明，IL-2和IFN-γ都不足以维持NK样活性，并提示需要其他分子。
