Musilová J, Michalová K, Pacovský V
Gerontology. 1984;30(6):365-70. doi: 10.1159/000212658.
The kinetics of cell proliferation and sister chromatid exchanges (SCE) were studied in blood lymphocytes treated by mitomycin C (MMC) in young and old human donors. In both the young and old donors MMC inhibited cell proliferation. Spontaneous SCE frequency was not age-dependent. In young donors, MMC induced a dose-related linear increase in SCE frequency. In old donors a significantly reduced SCE rate was induced by 80 ng/ml of MMC, suggesting an altered SCE response to mutagen. As SCE represent some form of DNA repair, the results can be interpreted as an indication of a defective DNA repair in aging human lymphocytes.
研究了丝裂霉素C（MMC）处理的年轻和老年人类供体血液淋巴细胞中的细胞增殖动力学和姐妹染色单体交换（SCE）情况。在年轻和老年供体中，MMC均抑制细胞增殖。自发SCE频率与年龄无关。在年轻供体中，MMC诱导SCE频率呈剂量相关的线性增加。在老年供体中，80 ng/ml的MMC诱导的SCE率显著降低，表明对诱变剂的SCE反应发生了改变。由于SCE代表某种形式的DNA修复，这些结果可解释为衰老人类淋巴细胞中DNA修复存在缺陷的一个迹象。
