Borra C, Cosmi G, Di Corcia A, Samperi R
J Chromatogr. 1984 Nov 9;311(1):9-15. doi: 10.1016/s0378-4347(00)84686-1.
In this reliable assay for determining the non-esterified long-chain fatty acid pattern in plasma, only 100 microliters of sample are needed and a single assay can be done within 40 min. The isolation procedure was performed by adsorption of fatty acids from plasma onto graphitized carbon black (Carbopack B) using a column method. After desorption and removal of the eluting phase, fatty acids are methylated by diazomethane and quantified by packed column gas chromatography. Analytical recoveries ranged between 91% and 103%. Within-run precision gave coefficients of variation of 2.3% and 11% for fatty acid concentrations of 58.2 and 0.6 mumol/l, respectively. Studies of plasma samples under various storage conditions indicated that reliable measurement of the non-esterified fatty acid fraction can be obtained even after 60 days if specimens are conserved at -18 degrees C in the presence of a suitable phospholipase inhibitor.
在这种用于测定血浆中非酯化长链脂肪酸模式的可靠分析方法中，仅需100微升样品，且一次分析可在40分钟内完成。分离过程通过柱法将血浆中的脂肪酸吸附到石墨化炭黑（Carbopack B）上进行。解吸并去除洗脱相后，脂肪酸用重氮甲烷甲基化，并用填充柱气相色谱法定量。分析回收率在91%至103%之间。批内精密度方面，脂肪酸浓度为58.2和0.6微摩尔/升时的变异系数分别为2.3%和11%。对不同储存条件下血浆样品的研究表明，如果样品在合适的磷脂酶抑制剂存在下于-18℃保存，即使60天后也能获得可靠的非酯化脂肪酸部分测量结果。
