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一种用于定量检测抗爱泼斯坦-巴尔病毒膜抗原gp340抗体的高灵敏度酶联免疫吸附测定法。

A highly sensitive enzyme-linked immunosorbent assay to quantitate antibodies to Epstein-Barr virus membrane antigen gp340.

作者信息

Randle B J, Epstein M A

出版信息

J Virol Methods. 1984 Nov;9(3):201-8. doi: 10.1016/0166-0934(84)90026-0.

Abstract

An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of antibodies to Epstein-Barr (EB) virus membrane antigen (MA) glycoprotein, gp340, in tamarins. The assay was found to be a thousand-fold more sensitive than conventional indirect immunofluorescence tests and consequently it was possible to follow accurately the sequential production of specific antibodies to gp340 by tamarins during a course of immunization.

摘要

已开发出一种酶联免疫吸附测定法(ELISA),用于检测绢毛猴体内针对爱泼斯坦-巴尔(EB)病毒膜抗原(MA)糖蛋白gp340的抗体。结果发现该测定法比传统的间接免疫荧光试验灵敏一千倍,因此在免疫过程中能够准确追踪绢毛猴体内针对gp340的特异性抗体的连续产生情况。

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