Witte S, Zenzes-Geprägs S
Res Exp Med (Berl). 1978 Jan 30;172(1):83-96. doi: 10.1007/BF01851068.
The extravascular protein contents in the perivascular connective tissue of a rat's mesenterial plate was measured ultramicrospectrophotometrically in situ and in vivo after changing the hydrostatic and colloidosmotic pressures of the blood. We analized the perivascular area of the different microcirculatory vessels, arterioles, capillaries and venules. The perivascular protein contents, which corresponds besides arterioles to 40% and besides venules to 59% in comparison to the intravascular blood plasma, decreased in a comparison group under the osmotic influence of the perfusion liquid on the mesenterial plate. An injection of an isotonic saline solution is followed only by small fluctuations of the perivascular protein contents during one hour. After blood loss a transitional increase of extravascular protein at the perivenular area was observed indicating the mobilisation of protein depots. Around arterioles the extravascular protein contents did not change significantly. An intravenous injection of albumin solution was followed by a short-termed increase of tissue protein around the arterioles. Around the venules after 50 minutes the extravascular protein contents increased significantly. Within one hour after the changes of the permeability conditions the maximal induceable protein movements in the perivascular space were calculated as +/- 1.35 g% plasma protein concentration corresponding to a maximun protein exchange of +/- 3.1 mg ml(-1) tissue.
在改变血液的流体静压和胶体渗透压后，采用超微量分光光度法在原位和体内测量大鼠肠系膜板血管周围结缔组织中的血管外蛋白质含量。我们分析了不同微循环血管（小动脉、毛细血管和小静脉）的血管周围区域。与血管内血浆相比，除小动脉外血管周围蛋白质含量为40%，除小静脉外为59%，在灌注液对肠系膜板的渗透影响下，对照组的该含量降低。注射等渗盐溶液后，一小时内血管周围蛋白质含量仅有小的波动。失血后，在小静脉周围区域观察到血管外蛋白质短暂增加，表明蛋白质储备的动员。在小动脉周围，血管外蛋白质含量没有明显变化。静脉注射白蛋白溶液后，小动脉周围组织蛋白质短暂增加。50分钟后，小静脉周围的血管外蛋白质含量显著增加。在渗透性条件改变后的一小时内，血管周围空间中最大可诱导的蛋白质移动量计算为±1.35 g%血浆蛋白浓度，相当于最大蛋白质交换量为±3.1 mg ml(-1)组织。
