Saikumar P, Kurup C K
Biochem J. 1984 Dec 15;224(3):955-60. doi: 10.1042/bj2240955.
Administration of 2-methyl-4-dimethylaminobenzene in the diet (0.1%, w/w) for 85-90 days doubled the content of mitochondria in the livers of rats. The azodye was covalently bound to liver proteins, and about 15% of the amount found in liver was associated with the mitochondrial fraction. Mitochondria isolated from the livers of azodye-fed animals showed drastically lowered ability to oxidize NAD+-linked substrates. The inhibited electron-transfer step was the reduction of ubiquinone. The organelles showed a large increase in succinate oxidase activity. The activity of cytochrome oxidase and the content of cytochrome aa3 were substantially higher in these organelles. Azodye-fed animals showed depressed serum cholesterol concentrations. The content of ubiquinone in liver also registered a small increase.
在大鼠饮食中添加0.1%（w/w）的2-甲基-4-二甲基氨基苯，持续85 - 90天，可使大鼠肝脏中的线粒体含量增加一倍。偶氮染料与肝脏蛋白质共价结合，肝脏中发现的该染料约15%与线粒体部分相关。从喂食偶氮染料的动物肝脏中分离出的线粒体氧化NAD⁺连接底物的能力大幅降低。受抑制的电子传递步骤是泛醌的还原。这些细胞器的琥珀酸氧化酶活性大幅增加。这些细胞器中细胞色素氧化酶的活性和细胞色素aa3的含量显著更高。喂食偶氮染料的动物血清胆固醇浓度降低。肝脏中泛醌的含量也略有增加。
