Reddy C C, Rao M K, Mastro A M, Egan R W
Biochem Int. 1984 Dec;9(6):755-61.
Rat basophilic leukemia cells have frequently been employed for investigating the pathways of leukotriene biosynthesis, a class of biologically active arachidonic acid metabolites. However, information is lacking on the levels of selenium-dependent glutathione peroxidase (Se-GSH-Px), non-Se-GSH-Px and glutathione S-transferases (GSH-S-Trs), key enzymes involved in fatty acid hydroperoxide metabolism and leukotriene biosynthesis in these cells. Both GSH-S-Trs and non-Se-GSH-Px reactions are catalyzed by the same enzyme. In the present studies, we have measured the enzyme activities of GSH-Px(s) and GSH-S-Trs in the 105,000 X g supernatant fraction of sonified RBL-1 cells. The specific activities for GSH-Px(s) toward H2O2, cumene hydroperoxide, and 15S-hydroperoxy-eicosatetraenoic acid (15S-HPETE) are 12.6, 17.9 and 26.9 nmoles X min-1 X mg-1 protein, respectively. A specific activity of 18.9 nmoles X min-1 X mg-1 protein with 1-chloro-2,4-dinitrobenzene was estimated for the GSH-S-Trs. Therefore, the cell fraction that exhibits 5-lipoxygenase activity also contains selenium and non-selenium glutathione peroxidases.
大鼠嗜碱性白血病细胞经常被用于研究白三烯生物合成途径，白三烯是一类具有生物活性的花生四烯酸代谢产物。然而，关于这些细胞中参与脂肪酸氢过氧化物代谢和白三烯生物合成的关键酶——硒依赖性谷胱甘肽过氧化物酶（Se-GSH-Px）、非Se-GSH-Px和谷胱甘肽S-转移酶（GSH-S-Trs）的水平，目前还缺乏相关信息。GSH-S-Trs和非Se-GSH-Px反应由同一种酶催化。在本研究中，我们测定了超声处理的RBL-1细胞105,000×g上清液中GSH-Px(s)和GSH-S-Trs的酶活性。GSH-Px(s)对过氧化氢、氢过氧化苯异丙酯和15S-氢过氧化二十碳四烯酸（15S-HPETE）的比活性分别为12.6、17.9和26.9纳摩尔×分钟-1×毫克-1蛋白质。GSH-S-Trs对1-氯-2,4-二硝基苯的比活性估计为18.9纳摩尔×分钟-1×毫克-1蛋白质。因此，表现出5-脂氧合酶活性的细胞组分也含有硒和非硒谷胱甘肽过氧化物酶。
