Young B W, Podesta R B
J Parasitol. 1984 Dec;70(6):931-6.
Initial uptake of choline by Schistosoma mansoni (2-min uptake) revealed no differences between male, female or paired worms for any of the control or experimental groups. After a 30-min uptake period, however, males showed significantly higher uptake of choline in the presence of mitomycin C, cytochalasin B and calcium ionophore A23187, while paired worms showed significantly reduced uptake in the presence of actinomycin D, puromycin, mitomycin C, cytochalasin B, colchicine, insulin, thyroxine and lysine. Choline uptake by females was elevated, in the presence of cytochalasin B at 30 min, although not significantly. Significantly increased incorporation of choline into phosphatidylcholine was observed following a 30-min incubation with 5-hydroxytryptamine (males), puromycin and thyroxine (females) and calcium ionophore A23187 (males, females and pairs). These effects on phosphatidylcholine synthesis are discussed in relation to the uptake data and to previous work concerning the outer membrane complex of the parasite as an important facet of parasite resistance to the host immune response.
曼氏血吸虫对胆碱的初始摄取（2分钟摄取）显示，在任何对照组或实验组中，雄虫、雌虫或配对虫之间均无差异。然而，在摄取30分钟后，在丝裂霉素C、细胞松弛素B和钙离子载体A23187存在的情况下，雄虫对胆碱的摄取显著增加，而在放线菌素D、嘌呤霉素、丝裂霉素C、细胞松弛素B、秋水仙碱、胰岛素、甲状腺素和赖氨酸存在的情况下，配对虫的摄取显著减少。在30分钟时，雌虫在细胞松弛素B存在的情况下对胆碱的摄取有所升高，尽管不显著。在与5-羟色胺（雄虫）、嘌呤霉素和甲状腺素（雌虫）以及钙离子载体A23187（雄虫、雌虫和配对虫）孵育30分钟后，观察到胆碱掺入磷脂酰胆碱的量显著增加。结合摄取数据以及先前关于寄生虫外膜复合物作为寄生虫抵抗宿主免疫反应重要方面的研究工作，对这些对磷脂酰胆碱合成的影响进行了讨论。
