Hoffmann D, Van Regenmortel M H
J Mol Evol. 1984;21(1):14-8. doi: 10.1007/BF02100623.
We used an indirect enzyme-linked immunosorbent assay (ELISA) for measuring the immunological cross-reactivities between bird lysozymes and a lysozyme isolated from the blood of the insect Locusta migratoria. The degrees of cross-reactivity among five avian lysozymes measured by ELISA agreed approximately with those observed in earlier work using microcomplement fixation tests. This latter technique is not suitable for detecting immunological cross-reactivity between proteins that differ in sequence by more than 30%-40%. In contrast, ELISA is able to detect distant relationships between antigens such as lysozymes that differ in sequence by as much as 60%. It seems likely that the use of ELISA procedures will extend the range of homologous proteins that can be compared by immunochemical means.
我们使用间接酶联免疫吸附测定法（ELISA）来测量鸟类溶菌酶与从昆虫飞蝗血液中分离出的一种溶菌酶之间的免疫交叉反应性。通过ELISA测量的五种禽类溶菌酶之间的交叉反应程度与早期使用微量补体固定试验观察到的结果大致相符。后一种技术不适用于检测序列差异超过30%-40%的蛋白质之间的免疫交叉反应性。相比之下，ELISA能够检测出序列差异高达60%的抗原（如溶菌酶）之间的远缘关系。使用ELISA程序似乎有可能扩大可以通过免疫化学方法进行比较的同源蛋白质的范围。
