Wu Y C, Hsieh R P, Lü Y C
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1984 Nov;17(4):177-87.
To clarify the mechanism of immunotoxicity of polychlorinated biphenyls (PCB), delayed type hypersensitivity skin test, lymphoproliferative responses, and lymphocyte subpopulations defined by monoclonal antibodies were performed on 30 patients and 38 age/sex-matched healthy individuals. Three years ago the patients had ingested rice bran oil accidentally contaminated by PCB. Except for lymphocyte proliferation, the chronic effect of PCB and its derivatives upon cell-mediated immunity (CMI) in these patients was not so evident when compared to that found within one year of exposure. Delayed type hypersensitivity skin response to purified protein derivative (PPD) and the numbers of peripheral T and B lymphocytes were within normal range. The enumeration of lymphocyte subsets demonstrated decreased T4 (inducer/helper) cells and increased T8 (cytotoxic/suppressor) cells. The abnormally augmentated spontaneous proliferation and proliferation responses to phytohemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM) and PPD implicated that both T and B lymphocytes were functionally affected. This may well be the result of the immunotoxicity rather than the enhancement of cell-mediated immunity by these toxic compounds. While there was no correlation between the results of those assays and blood PCB levels, it appears that the lymphoproliferative assay may be applied as a sensitive test in reflecting the severity of PCB immunotoxicity.
为阐明多氯联苯（PCB）的免疫毒性机制，对30例患者和38名年龄及性别匹配的健康个体进行了迟发型超敏皮肤试验、淋巴细胞增殖反应以及用单克隆抗体鉴定淋巴细胞亚群。三年前这些患者意外摄入了被PCB污染的米糠油。除淋巴细胞增殖外，与暴露后一年内的情况相比，PCB及其衍生物对这些患者细胞介导免疫（CMI）的慢性影响并不明显。对纯化蛋白衍生物（PPD）的迟发型超敏皮肤反应以及外周T和B淋巴细胞数量均在正常范围内。淋巴细胞亚群计数显示T4（诱导/辅助）细胞减少，T8（细胞毒性/抑制）细胞增加。对植物血凝素（PHA）、刀豆蛋白A（Con A）、商陆有丝分裂原（PWM）和PPD的自发增殖和增殖反应异常增强，这表明T和B淋巴细胞的功能均受到影响。这很可能是免疫毒性的结果，而非这些有毒化合物增强了细胞介导免疫。虽然这些检测结果与血液中PCB水平之间没有相关性，但淋巴细胞增殖试验似乎可作为反映PCB免疫毒性严重程度的敏感检测方法。
