Pomar F T, Meinardi C A, Bacca J A
Departamento de Microbiología y Enzimología, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, Argentina.
Rev Argent Microbiol. 1983;15(1):9-17.
The search of a microorganism with the ability to produce the enzyme beta-galactosidase was undertaken according with the requirements of the market, in economical, technological and sanitary terms. The process consisted of recovery and use of the effluent from milk and cheese used to feed pigs, producing at the same time different types of contamination; once investigated and adjusted the technological variables to produce the enzyme, and selected the most convenient microorganism for such purpose the acting upon the extraction, conservation and purification of the product were adjusted. Comparative results of conversion were obtained with different test, at laboratory scale and industrial plants, in similar conditions to those obtained with importation products.
根据市场在经济、技术和卫生方面的要求,开展了对具有产生β-半乳糖苷酶能力的微生物的搜索。该过程包括回收和利用用于喂猪的牛奶和奶酪废水,同时产生不同类型的污染物;一旦研究并调整了生产该酶的工艺变量,并选择了最适合此目的的微生物,就对产品的提取、保存和纯化进行了调整。在实验室规模和工业工厂中,在与进口产品相似的条件下,通过不同测试获得了转化的比较结果。