Devilee P, Warnaar J N, Giphart M J
Department of Immunohaematology, University Medical Center, Leiden, The Netherlands.
Exp Clin Immunogenet. 1984;1(2):90-8.
Considerable homology concerning the major histocompatibility complexes of man, rhesus monkey, dog, cat, cow, sheep, rabbit and goat has been established by Southern blot analyses. Human cDNAs from both class I and class II gene products were used as probes. Besides the expected hybridization of the probes to human DNA, the probes displayed a clear affinity for the other DNAs as well. Equal numbers of bands-about 16 depending on the enzyme used - appear in digests of human and rhesus monkey DNA when the class I cDNA is used as a probe. This number is slightly smaller and varying in the other species. The same holds for class II probes. Both class I and class II probes show no detectable homology at all with Xenopus DNA under the conditions used. A substantial MHC homology at the DNA level between man and rhesus monkey can be concluded from these data, this homology being somewhat smaller in various other species. Further implications for the evolution of MHC are discussed.
通过Southern印迹分析已确定，人类、恒河猴、狗、猫、牛、羊、兔和山羊的主要组织相容性复合体存在相当程度的同源性。来自I类和II类基因产物的人类cDNA用作探针。除了探针与人类DNA的预期杂交外，探针也对其他DNA显示出明显的亲和力。当使用I类cDNA作为探针时，人类和恒河猴DNA消化产物中出现的条带数量相等——根据所用酶的不同约为16条。这个数字在其他物种中略小且有所变化。II类探针也是如此。在所使用的条件下，I类和II类探针与非洲爪蟾DNA均未显示出可检测到的同源性。从这些数据可以得出，人类和恒河猴在DNA水平上存在相当大的MHC同源性，这种同源性在其他各种物种中略小。文中还讨论了MHC进化的进一步影响。
