Maryanski J L, MacDonald H R, Cerottini J C
J Immunol. 1980 Jan;124(1):42-7.
A sensitive limiting dilution microculture system was used to obtain minimal estimates of the frequency of cytolytic T lymphocyte precursor cells (CTL-P) directed against DBA/2 alloantigens, after priming of spleen cells in unidirectional mixed leukocyte cultures (MLC, C57BL/6 anti-DBA/2). The mean CTL-P frequency in day 4 to 5 MLC populations was found to be approximately 50- to 100-fold greater than the frequency in normal spleen, and up to 25% of the cells present in such MLC could be identified operationally as CTL-P. Even higher frequencies (up to 50%) of CTL-P were obtained in a population of large-sized cells separated from day 4 MLC by velocity sedimentation. Furthermore, since a strikingly quantitative correlation was observed between CTL activity and CTL-P frequency in such separated MLC populations, it is likely that mature CTL in MLC are not end cells, but can further proliferate and thus behave operationally as CTL-P.
在单向混合淋巴细胞培养（MLC，C57BL/6 抗 DBA/2）中对脾细胞进行致敏后，使用一种灵敏的有限稀释微量培养系统来获得针对 DBA/2 同种异体抗原的细胞毒性 T 淋巴细胞前体细胞（CTL-P）频率的最低估计值。发现在第 4 至 5 天的 MLC 群体中，CTL-P 的平均频率比正常脾中的频率大约高 50 至 100 倍，并且此类 MLC 中多达 25%的细胞在操作上可被鉴定为 CTL-P。通过速度沉降从第 4 天的 MLC 中分离出的大细胞群体中获得了更高频率（高达 50%）的 CTL-P。此外，由于在这种分离的 MLC 群体中观察到 CTL 活性与 CTL-P 频率之间存在显著的定量相关性，因此 MLC 中的成熟 CTL 可能不是终末细胞，而是可以进一步增殖，从而在操作上表现为 CTL-P。
