Degiovanni G, Schaaf-Lafontaine N
J Immunol. 1981 Feb;126(2):641-7.
The inhibitory effect of spleen cells, precultured in the presence of FCS, was assayed on the memory cytolytic T lymphocytes (CTL) response to alloantigens. For this, we have used in vitro conditions in which both particulate alloantigen and MLC SN are required to allow the generation of CTL. It was shown that the CTL response was totally inhibited in the presence of 5 to 7 days precultured spleen cells. This inhibitory effect was partly due to removal, by those precultured cells, of relevant factor(s) contained in the MLC SN. After velocity sedimentation at unit gravity, it was shown that the T cells able to inhibit the cytolytic response and to remove MLC SN factor(s) are found in the fractions containing the large proliferating cells. It was further demonstrated that in the presence of inhibiting cells, a significant CTL response may be obtained after addition of concentrated MLC SN. However, in this way, this inhibitory effect was not totally circumvented, which suggests that the memory CTL response is also impaired by other mechanisms.
在含有胎牛血清（FCS）的条件下预培养的脾细胞，对同种异体抗原的记忆性细胞毒性T淋巴细胞（CTL）反应的抑制作用进行了测定。为此，我们采用了体外条件，其中颗粒性同种异体抗原和混合淋巴细胞培养上清液（MLC SN）都是产生CTL所必需的。结果表明，在存在预培养5至7天的脾细胞时，CTL反应被完全抑制。这种抑制作用部分是由于那些预培养细胞去除了MLC SN中所含的相关因子。在单位重力下进行速度沉降后，发现能够抑制细胞溶解反应并去除MLC SN因子的T细胞存在于含有大量增殖细胞的组分中。进一步证明，在存在抑制细胞的情况下，加入浓缩的MLC SN后可获得显著的CTL反应。然而，通过这种方式，这种抑制作用并未被完全克服，这表明记忆性CTL反应也受到其他机制的损害。
