Craig N L, Roberts J W
Nature. 1980 Jan 3;283(5742):26-30. doi: 10.1038/283026a0.
The recA protein mediates both genetic recombination and several cellular responses to DNA damage, including the induction of temperate bacteriophage. Indication of phage lambda results from proteolytic cleavage of lambda repressor directed by recA protein. We show here that this cleavage reaction requires both polynucleotide and ATP. We suggest that a stoichiometric complex of recA protein and DNA is active both to destroy repressors by proteolytic cleavage and to initiate pairing of this DNA to its homologous sequence in a DNA duplex ('strand invasion').
RecA蛋白介导基因重组以及细胞对DNA损伤的多种反应，包括温和噬菌体的诱导。λ噬菌体的诱导是由RecA蛋白指导的λ阻遏物的蛋白水解切割所致。我们在此表明，这种切割反应需要多核苷酸和ATP。我们认为，RecA蛋白与DNA的化学计量复合物既能通过蛋白水解切割破坏阻遏物，又能启动该DNA与DNA双链体中同源序列的配对（“链侵入”）。
