Walsh M P, Vallet B, Cavadore J C, Demaille J G
J Biol Chem. 1980 Jan 25;255(2):335-7.
In order to identify the physiological regulator of calcium dependent myosin light chain kinases of cardiac, skeletal, and smooth muscles, the effects of the three homologous calciproteins, calmodulin, troponin C, and parvalbumin, on the kinases isolated from bovine myocardium, rabbit skeletal muscle, and turkey gizzard were examined. Only calmodulin was effective in stimulating the cardiac, skeletal, or smooth muscle kinase; troponin C and parvalbumin exhibited no activation of any of the three kinases, even when examined at concentrations as high as 10-(5) M. It is concluded that calmodulin is the specific regulator of myosin light chain kinase in cardiac, skeletal, and smooth muscle.
为了鉴定心脏、骨骼肌和平滑肌中钙依赖性肌球蛋白轻链激酶的生理调节因子，研究了三种同源钙结合蛋白，即钙调蛋白、肌钙蛋白C和小白蛋白，对从牛心肌、兔骨骼肌和火鸡砂囊中分离出的激酶的影响。只有钙调蛋白能有效刺激心脏、骨骼肌或平滑肌激酶；肌钙蛋白C和小白蛋白即使在高达10-(5)M的浓度下检测，也未表现出对这三种激酶中任何一种的激活作用。得出的结论是，钙调蛋白是心脏、骨骼肌和平滑肌中肌球蛋白轻链激酶的特异性调节因子。
